A2556

Monoklonaler Anti-Kaninchen-IgG-Antikörper (γ-kettenspezifisch)

clone RG-96, purified immunoglobulin, buffered aqueous glycerol solution

• Synonym(e): Monoclonal Anti-Rabbit IgG (γ-chain specific)
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: alkaline phosphatase conjugate
• Clone: RG-96, monoclonal
• Application: ELISA (d), IHC (p), WB
• Citations: 27

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: purified immunoglobulin
• antibody product type: secondary antibodies
• clone: RG-96, monoclonal
• form: buffered aqueous glycerol solution
• species reactivity: rabbit
• should not react with: goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken
• technique(s): direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Beschreibung

General description

Das Produkt erkennt ein Epitop, das sich an der γ-(Schwer-)Kette von Kaninchen-IgG befindet. Beim Immunblotting erkennt der Antikörper sowohl native als auch denaturierte Formen von Kaninchen-IgG. In ELISA ist der Antikörper spezifisch für Kaninchen-IgG und zeigt keine Kreuzreaktivität mit Kaninchen-IgA und -IgM oder Human-IgG, -IgA und -IgM. Es wurde keine Kreuzreaktion mit IgG von folgenden Spezies festgestellt: Rind, Katze, Huhn, Hund, Ziege, Meerschweinchen, Pferd, Schwein, Ratte oder Schaf.

Monoclonal Anti-Rabbit IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE.

Application

Monoclonal Anti-Rabbit IgG (γ-chain specific)Alkaline Phosphatase antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 2
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)