ZRB1313

Anti-Cyclin D1 Antibody, clone 1J9, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, G1/S-specific cyclin-D1, PRAD1 oncogene
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 1J9, monoclonal, recombinant monoclonal
• Application: ICC, IHC (p), WB
• Citations: -

Eigenschaften

• biological source: rabbit (recombinant)
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1J9, monoclonal, recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 33.73 kDa, observed mol wt ~38 kDa
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• isotype: IgG
• UniProt accession no.: P24385
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CCND1(595)

Beschreibung

General description

G1/S-specific cyclin-D1 (UniProt: P24385; also known as B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene) is encoded by the CCND1 (also known as BCL1, PRAD1) gene (Gene ID: 595) in human. Cyclin D1 is an considered to be an essential regulator of the G1-S transition in response to growth factor stimulation in somatic cells. Together with cyclin dependent kinases 4 and 6 (CDK4 and CDK6), it forms active complexes that are shown to promote cell cycle progression by phosphorylating and inactivating the retinoblastoma protein (RB). Phosphorylation of RB1 allows dissociation of E2F from the RB/E2F complex and the subsequent transcription of E2F target genes that are responsible for the progression through the G1 phase. Cyclin D1 levels are reported to rise early in G1 and continue to accumulate until the G1/S-phase boundary when its levels rapidly decline. It exhibits a short half-life of about 24 minutes and is readily degraded in the 26S proteasome. Cyclin D1 is shown to repress DNA replication by binding to proliferating cell nuclear antigen (PCNA) and Cdk2. The binding of cyclin D1 to PCNA directly inhibits DNA synthesis. The overexpression of cyclin D1 and its deregulated degradation have been linked to the development and progression of various cancers. A chromosomal aberration involving CCND1 gene is reported to cause B-lymphocytic malignancy. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Alao, JP (2007). Mol. Cancer 6: 24; Albero, R., et al. (2018). J. Clin. Invest. 128(9); 4132-4147).

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human Cyclin D1.

Application

Anti-Cyclin D1, clone 1J9 ZooMAb, Cat. No. ZRB1313, is a recombinant rabbit monoclonal antibody that specifically detects Cyclin D1 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:10 dilution from a representative lot detected Cyclin D1 in human pancreatic cancer tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cyclin D1 in HeLa, A431, HUVEC, MCF-7, SK-MEL-28 and HT-29 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

Clone 1J9 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Cyclin D1. It targets an epitope within 19 amino acids from the C-terminal half.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable