ZRB1490

Anti-CDK6 Antibody, clone 2J3 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): Cell division protein kinase 6, Cyclin-dependent kinase 6, Serine/threonine-protein kinase PLSTIR
• NACRES: NA.43
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 2J3, monoclonal, recombinant monoclonal
• Application: ICC, IHC (p), WB, affinity binding assay
• Citations: -

Eigenschaften

• biological source: rabbit (recombinant)
• Quality Segment: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2J3, monoclonal, recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 36.94 kDa, observed mol wt 37 kDa
• species reactivity: human, rat, mouse
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Sustainability features
• technique(s): affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• isotype: IgG
• UniProt accession no.: Q00534
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CDK6(1021)

Beschreibung

General description

Cyclin-dependent kinase 6 (UniProt: Q00534; also known as EC:2.7.11.22, Cell division protein kinase 6, Cdk6, Serine/threonine-protein kinase PLSTIRE) is encoded by the CDK6 (also known as CDKN6) gene (Gene ID: 1021) in human. CDK6 is a ubiquitously expressed serine/threonine-protein kinase that is involved in the control of the cell cycle and differentiation. Its kinase domain is localized to amino acids 13-300 and its kinase activity is observed only in the nucleus. It associates with D-type cyclins (D1, D2 and D3), and the resulting complexes enable the cell to pass from the G1 phase to the S phase. Its binding to cyclin promotes its activation by phosphorylation at threonine 177. CDK6 is considered as a versatile player in transcriptional regulation and differentiation, participating in the integration of signaling networks and controlling changes in gene expression and epigenetic status, which contribute to cell fate determination. Over-expression of CDK6 has been reported in some leukemias and malignancies, including sarcoma, glioma, breast tumors, lymphoma, and melanoma. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tigan., AS et al. (2016). Oncogene 35(24); 3083-3091).

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

This product aligns with one or more of the 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human CDK6.

Application

Anti-CDK6, clone 2J3 ZooMAb, Cat. No. ZRB1490, is a recombinant Rabbit monoclonal antibody that specifically targets CDK6 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CDK6 in HeLa cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound CDK6 peptide with a KD value of 2.8 x 10-6 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CDK6 in HeLa, A431, HUVEC, and NIH 3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CDK6 in human kidney and human testis tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Biochem/physiol Actions

Clone 2J3 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects CDK6. It targets an epitope within 21 amino acids from the C-terminal region.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 2