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Abberior® STAR 635P, Azid
suitable for fluorescence
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Über diesen Artikel
NACRES:
NA.32
UNSPSC Code:
12352200
form
solid
solubility
DMF: 0.25 mg/mL, clear
fluorescence
λex 634 nm; λem 654.0 nm±15 nm in PBS (pH 7.1)
suitability
suitable for fluorescence
storage temp.
−20°C
General description
Abberior STAR635P is the first member of an entirely new class of dyes introduced exclusively by Abberior - first commercially available phosphorylated fluorescent dye. The dye excels in its photophysical parameters, e.g. very bright, very stable and it enables basically background free imaging.
Abberior STAR 635P can substitute Atto™ 647N, AlexaFluor 647, or Cy5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength around 750 nm is recommended. Please see reference1 for detailled characteristics.
Best results are obtained with freshly prepared samples.
Abberior STAR 635P particularly well suits the Leica TCS STED system and delivers high-resolution STED images with unmatched quality, e.g. extremely low/no background. The dye serves as an ideal partner for several 2-color STED packages.
Key Features
Absorption Maximum, λmax: 633 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 75′000 M-1cm-1 (water)
Correction Factor, CF260 = ε260/εmax: 0.42
Correction Factor, CF280 = ε280/εmax: 0.47
Fluorescence Maximum, λfl: 653 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 740 - 770 nm
Fluorescence Quantum Yield, η: 0.92 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.3 (PBS, pH 7.4)
Abberior STAR 635P can substitute Atto™ 647N, AlexaFluor 647, or Cy5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength around 750 nm is recommended. Please see reference1 for detailled characteristics.
Best results are obtained with freshly prepared samples.
Abberior STAR 635P particularly well suits the Leica TCS STED system and delivers high-resolution STED images with unmatched quality, e.g. extremely low/no background. The dye serves as an ideal partner for several 2-color STED packages.
Key Features
- First phosphorylated dye
- Unmatched, background free STED imaging contrast
- Verified in the Leica STED system
Absorption Maximum, λmax: 633 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 75′000 M-1cm-1 (water)
Correction Factor, CF260 = ε260/εmax: 0.42
Correction Factor, CF280 = ε280/εmax: 0.47
Fluorescence Maximum, λfl: 653 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 740 - 770 nm
Fluorescence Quantum Yield, η: 0.92 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.3 (PBS, pH 7.4)
Application
Designed and tested for fluorescent super-resolution microscopy
Other Notes
Legal Information
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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