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49429
Phalloidin-Atto 532
suitable for fluorescence, ≥90.0% (HPLC)
Synonym(e):
Atto 532-Phalloidin
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 10 nmol | Warenkorb auf Verfügbarkeit prüfen | € 474,00 |
Über diesen Artikel
NACRES:
NA.32
UNSPSC Code:
12352108
Technischer Dienst
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Unterstützung erhaltenQuality Level
assay
≥90.0% (HPLC)
manufacturer/tradename
ATTO-TEC GmbH
λ
in acetonitrile: water (1:9) (with 0.01% trifluoroacetic acid)
UV absorption
λ: 534-540 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
General description
Atto 532 is a novel fluorescent label related to the well-known dye Rhodamine 6G. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, excellent water solubility, and very little triplet formation. After coupling to a substrate Atto 532 Phalloidin carries a net electrical charge of -1. Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10–8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours.
Application
Fluorescent conjugates of phalloidin, rhodamine-phalloidin staining reagents, such as Phalloidin–Atto 532 are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
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Dieser Artikel | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| assay ≥90.0% (HPLC) | assay ≥90.0% (HPLC) | assay ≥90.0% (sum of isomers, HPLC) | assay ≥90% (HPLC) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH |
| UV absorption λ: 534-540 nm Amax | UV absorption λ: 600-606 nm Amax | UV absorption λ: 651- 657 nm Amax | UV absorption λ: 429-435 nm Amax |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Verwandter Inhalt
Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.
J E Bowe et al.
Diabetologia, 56(4), 783-791 (2013-01-25)
Glucose plays two distinct roles in regulating insulin secretion from beta cells--an initiatory role, and a permissive role enabling receptor-operated secretagogues to potentiate glucose-induced insulin secretion. The molecular mechanisms underlying the permissive effects of glucose on receptor-operated insulin secretion remain
Models of the collective behavior of proteins in cells: tubulin, actin and motor proteins.
Tuszynski JA, Brown JA, Sept D.
J. Biol. Physics, 29, 401-428 (2003)
Magdalena Izdebska et al.
Acta histochemica, 115(5), 487-495 (2013-01-15)
Quantum dots (QDs) are fluorescent nanocrystals whose unique properties are fundamentally different from organic fluorophores. Moreover, their cores display sufficient electron density to be visible under transmission electron microscopy (TEM). Here, we report a technique for phalloidin-based TEM detection of
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 49429-10NMOL | 04061832532233 |