171R-1

CD71 (EP232) Rabbit Monoclonal Primary Antibody

• Synonym(e): TFR1, TRF, TRFR, Transferrin Receptor 1, p90, transferrin receptor (p90, CD71)
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: EP232, monoclonal
• Application: IHC (p)
• Citations: 8

Eigenschaften

• biological source: rabbit
• Quality Level: 100, 500
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: EP232, monoclonal
• description: For In Vitro Diagnostic Use in Select Regions (See Chart)
• form: buffered aqueous solution
• species reactivity: human
• packaging: vial of 0.1 mL concentrate (171R-14), vial of 0.1 mL concentrate Research Use Only (171R-14-RUO), vial of 0.5 mL concentrate (171R-15), vial of 1.0 mL concentrate (171R-16), vial of 1.0 mL concentrate Research Use Only (171R-16-RUO), vial of 1.0 mL pre-dilute Research Use Only (171R-17-RUO), vial of 1.0 mL predilute ready-to-use (171R-17), vial of 7.0 mL pre-dilute ready-to-use Research Use Only (171R-18-RUO), vial of 7.0 mL predilute ready-to-use (171R-18)
• manufacturer/tradename: Cell Marque^®
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200 (concentrated)
• isotype: IgG
• control: bone marrow
• shipped in: wet ice
• storage temp.: 2-8°C
• visualization: cytoplasmic, membranous

Beschreibung

General description

Transferrin receptor 1 (CD71) is expressed on placental syncytiotrophoblasts, myocytes, basal keratinocytes, hepatocytes, endocrine pancreas, spermatocytes, and erythroid precursors. The level of transferrin receptor expression is highest in early erythroid precursors through the intermediate normoblast phase, after which expression decreases through the reticulocyte phase. The maturation of erythrocytes results in loss of transferrin receptor expression. AntiCD71 is useful in identifying erythroid precursors. The high level of transferrin receptor within erythroid precursors makes anti-CD71 an excellent marker for evaluation of erythroid precursors within bone marrow biopsy specimens and shows the following features: 1) distinct membranous and cytoplasmic staining pattern, which is easily recognized in bone marrow biopsy; 2) restriction to erythroid lineage within bone marrow biopsy specimens; 3) CD71 expression decreases with the maturation of erythrocytes, with the highest level seen in early forms and the lowest level in late normoblast stage, and most importantly; 4) mature erythrocytes do not express CD71.[1][2][3][4][5]

Physical form

Predilute: diluted in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
Concentrate: diluted in Phosphate Buffered Saline, pH 7.2, with 1% BSA and <0.1% Sodium Azide

Preparation Note

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Analysis Note

United States - IVD
Canada - IVD
European Union - IVD
Japan - RUO

Other Notes

CD71 Positive Control Slides, Product No. 171S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

For Technical Service please contact: 800-665-7284 or email: [email protected]

Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable