8-Anilino-1-naphthalinsulfonsäure Hemimagnesiumsalz Hydrat

Name: 8-Anilino-1-naphthalinsulfonsäure Hemimagnesiumsalz Hydrat
Brand: SIGMA
Price: 314 EUR

suitable for fluorescence, ≥95.0% (T)

Synonym(e):

ANSA, Magnesium-8-anilino-1-naphthalinsulfonat

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25 G

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Über diesen Artikel

Empirische Formel (Hill-System):

C₁₆H₁₂Mg_0.5NO₃S · xH₂O

CAS-Nummer:

18108-68-4

Molekulargewicht:

310.49 (anhydrous basis)

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

329749053

EC Number:

242-004-7

Beilstein/REAXYS Number:

4834623

MDL number:

MFCD16661224

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InChI key

FBANPIUXAXTAFX-UHFFFAOYSA-L

InChI

1S/2C16H13NO3S.Mg.H2O/c2*18-21(19,20)15-11-5-7-12-6-4-10-14(16(12)15)17-13-8-2-1-3-9-13;;/h2*1-11,17H,(H,18,19,20);;1H2/q;;+2;/p-2

SMILES string

O.[Mg++].[O-]S(=O)(=O)c1cccc2cccc(Nc3ccccc3)c12.[O-]S(=O)(=O)c4cccc5cccc(Nc6ccccc6)c45

assay

≥95.0% (T)

form

powder

technique(s)

titration: suitable

solubility

hot water: 50 mg/mL, green

λ_max

465-475 nm

fluorescence

λ_ex 388 nm; λ_em 470 nm in 0.1 M Tris, 0.2 M KCl, pH 9.0, BSA

suitability

suitable for fluorescence

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Dieser Artikel	A1028	A5144	10417
Sigma-Aldrich 10419 8-Anilino-1-naphthalenesulfonic acid hemimagnesium salt hydrate	Sigma-Aldrich A1028 8-Anilino-1-naphthalenesulfonic acid	Sigma-Aldrich A5144 8-Anilino-1-naphthalenesulfonic acid hemimagnesium salt hydrate	Sigma-Aldrich 10417 8-Anilino-1-naphthalenesulfonic acid ammonium salt
technique(s) titration: suitable	technique(s) -	technique(s) -	technique(s) -
grade for fluorescence	grade -	grade for fluorescence	grade for fluorescence
fluorescence λ_ex 388 nm; λ_em 470 nm in 0.1 M Tris, 0.2 M KCl, pH 9.0, BSA	fluorescence -	fluorescence -	fluorescence λ_ex 388 nm; λ_em 470 nm in 0.1 M Tris, 0.2 M KCl, pH 9.0, BSA
application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) -
solubility hot water: 50 mg/mL, green	solubility DMF: 50 mg/mL	solubility H₂O: 50 mg/mL	solubility H₂O: 0.1 g/5mL, clear to very slightly hazy (hot), NaOH: 1 N, H₂O: soluble, acetone: soluble, methanol: soluble
form powder	form powder	form powder	form solid

General description

ANSA (8-Anilino-1-naphthalenesulfonic acid) is a hydrophobic polarity sensitive fluorescent dye useful as a site probe to detect conformational changes in cell and micelle membranes and molecules such as proteins. ANSA is also used as a fluorescent probe for the estimation of the critical micelle concentration (CMC) of surfactants.

Features and Benefits

Fluoreszenzsonde für die Bestimmung der kritischen Mizellenbildungskonzentration (CMC) von Tensiden[1]

pictograms

GHS07

signalword

Warning

hcodes

H315,H319,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Die Dokumentenbibliothek aufrufen

The identification of hydrophobic sites on the surface of proteins using absorption difference spectroscopy of bromophenol blue.

M Bertsch et al.

Analytical biochemistry, 313(2), 187-195 (2003-02-28)

Hydrophobic sites on the surface of protein molecules are thought to have important functional roles. The identification of such sites can provide information about the function and mode of interaction with other cellular components. While the fluorescence enhancement of polarity-sensitive

Rapid fluorescent "staining" of nondenatured protein bands in agar and polyacrylamide gels.

S T Nerenberg et al.

Analytical biochemistry, 43(2), 564-574 (1971-10-01)

Detection of an unfolding intermediate in alpha-urease with enhanced affinity for ANSA.

C D Peters et al.

Biochemistry and cell biology = Biochimie et biologie cellulaire, 75(1), 55-61 (1997-01-01)

Protein aggregation is believed to be due to conformers that expose hydrophobic clusters that promote protein association. Such conformers can be detected using a fluorescent probe like 8-anilino 1-naphthalenesulfonic acid (ANSA). Here we show that urease exposed to 1.0 M

Metal-catalyzed oxidation of brain-derived neurotrophic factor (BDNF): selectivity and conformational consequences of histidine modification.

J L Jensen et al.

Cellular and molecular biology (Noisy-le-Grand, France), 46(3), 685-696 (2000-06-29)

We have studied the metal-catalyzed oxidation (MCO) of brain-derived neurotrophic factor (BDNF) with regard to target sites and potential conformational changes of the protein. The exposure of BDNF to three different levels of ascorbate/Cu(II)/O2 [20 microM Cu(II), 2 mM ascorbate

Low concentration of guanidine hydrochloride induces the formation of an aggregation-prone state in alpha-urease.

F-O McDuff et al.

Biochemistry and cell biology = Biochimie et biologie cellulaire, 82(2), 305-313 (2004-04-03)

Canavalia ensiformis (jack bean) alpha-urease is a hexameric protein characterized by a complex denaturation mechanism. In previous papers, we have shown that a hydrophobic 8-anilino-1-naphthalenesulfonic acid (ANSA) binding conformer could be populated in a moderate concentration of denaturant. This state

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