PPB021

SSPE Buffer

pH 7.4, pHast Pack^™, powder

• Synonym(e): 1X SSPE Hybridization buffer, 1X SSPE buffer, SSPE Hybridization buffer, Saline-Sodium Phosphate-EDTA Buffer
• NACRES: NA.25
• UNSPSC Code: 41105319

Eigenschaften

• product line: pHast Pack^™
• Quality Segment: 100
• form: powder
• pH: 7.4

Beschreibung

Application

Saline-Sodium Phosphate-EDTA is used as a hybridization buffer in Northern and Southern blotting. It is also used as a wash buffer in DNA-microarray technique.

Features and Benefits

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Save time and minimize effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Packaging

Foil pouches

Preparation Note

Contents of one pouch, when dissolved in 1000 mL of ultrapure water, will yield a 1× solution containing 150 mM NaCl, 10 mM NaH₂PO₄, 1 mM EDTA, pH 7.4 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1