This product has not been tested for use as a cell lysis agent. M-Clarity Quality Level 500 is necessary for pharmaceutical and regulated applications. Product S7705 is a cell lysis buffer, but its Quality Level is 100.
Fortfahren mit
C5070
CHAPS-Hydrat
BioXtra, ≥98% (TLC)
Synonym(e):
3-[(3-Cholamidopropyl)dimethylammonio]-1-propansulfonat Hydrat, CHAPS
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 1 g | Warenkorb auf Verfügbarkeit prüfen | € 137,00 | |
| 5 g | Warenkorb auf Verfügbarkeit prüfen | € 540,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C32H58N2O7S · xH2O
CAS-Nummer:
Molekulargewicht:
614.88 (anhydrous basis)
NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
12161900
MDL number:
Technischer Dienst
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Unterstützung erhaltendescription
zwitterionic
Quality Segment
product line
BioXtra
assay
≥98% (TLC)
form
powder
mol wt
614.88 g/mol (anhydrous basis)
technique(s)
protein purification: suitable, protein quantification: suitable
impurities
≤0.001% Phosphorus (P), ≤0.1% Insoluble matter, <0.04% DMF
ign. residue
≤0.1%
mp
157 °C (dec.) (lit.)
solubility
H2O: 0.1 M, clear, colorless
anion traces
chloride (Cl-): ≤0.05%
cation traces
Al: ≤0.0005%, Ca: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH4+: ≤0.05%, Na: ≤0.005%, Pb: ≤0.001%, Zn: ≤0.0005%
SMILES string
O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
InChI
1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1
InChI key
SJCUTFKCLFLIFE-JWTJKVBLSA-N
General description
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.
Application
- 10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.[1]
- 0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.
- HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.
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Dieser Artikel | |||
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| assay ≥98% (TLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay >99% (TLC) |
| technique(s) protein purification: suitable, protein quantification: suitable | technique(s) dialysis: suitable, electrophoresis: suitable | technique(s) electrophoresis: suitable, protein quantification: suitable | technique(s) protein quantification: suitable |
| impurities ≤0.001% Phosphorus (P), <0.04% DMF, ≤0.1% Insoluble matter | impurities <0.04% DMF | impurities <0.04% DMF | impurities - |
| Quality Level 400 | Quality Level 400 | Quality Level 200 | Quality Level - |
| anion traces chloride (Cl-): ≤0.05% | anion traces - | anion traces - | anion traces - |
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Zulassungslistungen
Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.
68-12-2
CAS No.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| C5070-1G | 04061833501733 |
| C5070-5G | 04061833501740 |
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Hi, Could CHAPS C5070 quality level 400 be used as a cell lysis agent at the harvest stage for a human biological therapeutic?
1 answer-
Helpful?
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