Fortfahren mit
C0537
5-Carboxyfluorescein
≥99% purity (HPLC), powder
Synonym(e):
5-FAM
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Über diesen Artikel
Empirische Formel (Hill-System):
C21H12O7
CAS-Nummer:
Molekulargewicht:
376.32
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Beilstein/REAXYS Number:
57037
Produktname
5-Carboxyfluorescein, ≥99% (HPLC)
Quality Level
assay
≥99% (HPLC)
form
powder
solubility
0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
SMILES string
OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
InChI
1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
InChI key
NJYVEMPWNAYQQN-UHFFFAOYSA-N
Application
5-Carboxyfluorescein has been used as an internal marker in capillary isotachophoresis to quantify the charge-based low density lipoprotein subfractions.
Biochem/physiol Actions
5-Carboxyfluorescein is used for the staining of cells to differentiate between live and dead cells. It helps in monitoring the integrity and intactness of the cell membrane. It is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product 5-carboxyfluorescein. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.
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Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| C0537-50MG | 04061833289181 |