ST1049

Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231)

liquid, clone MCP231, Calbiochem^®

• UNSPSC Code: 12352203
• Clone: MCP231, monoclonal
• Species reactivity: human, yeast, rat, rabbit, mouse
• Application: —
• Citations: 3

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: MCP231, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human, yeast, rat, rabbit, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PSMA1(5682)

Beschreibung

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myleloma cells. Recognizes the ~29-32 kDa α1, 2, 3, 5, 6 and 7 subunits of the 20S proteasome.

Recognizes α1, 2, 3, 5, 6 and 7 subunits of 20S proteasome in human placental proteasome preparations.

This Anti-20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits Antibody is validated for use in WB, IP, ELISA, IF, ICC and IHC for the detection of 20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits.

Immunogen

Epitope: within the prosbox I motif common to α subunits (TVWSPQGRLHQVEYAMEA)

dinitrophenylated proteasomes

Application

Immunoblotting (1:1000, chemiluminescence)
Immunoprecipitation (not recommended)
ELISA (see comments)
Immunofluorescence (see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Frozen Sections (see application references)

Analysis Note

Positive Control
Human placental tissue

Other Notes

Immunoblotting using a human placental proteasome preparation yields two bands at ~29 kDa and ~32 kDa. In ELISA the antibody reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to α-type subunits. Antibody should be titrated for optimal results in individual systems.

Kopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA94, 2939.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Highly Toxic (H)

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable