See below for the preparation and usage instructions for this product.
Preparation of Reagent and Cell Staining
Before opening the vial, spin down the solid to the bottom by a microcentrifuge or by a desktop centrifuge.
1. To prepare a stock solution, dissolve the BioTracker 560 Orange Lysosome Dye 10 µg in 13.4 µL of distilled water or DMSO. It is not recommended to store DMSO solution of dye because this dye is not stable in DMSO.
2. Dilute the stock solution with medium to prepare 1 µM stain solution.
3. Remove the culture medium from cell culture dish and wash twice with medium.
Caution : Glass bottom dish etc. are recommended as cell culture dish, because it shows a low autofluorescence.
4. Add stain solution to the dish and incubate for 1 to 2 hours at 37 ?, 5% CO2. Even though the degree of stained
cells is varied with cell type or growth condition, usually cells are well stained around 2 hours.
5. After staining, wash once with medium and twice with HBSS buffer. Replace to HBSS buffer and observe the cells using a fluorescence microscopy.