SCC297

N2A Psen1/Psen2 Double Knockout Mouse Neuroblastoma Cell Line

Mouse

• UNSPSC Code: 41106514
• NACRES: NA.81
• Biological source: mouse
• Growth mode: N/A
• Manufacturer/tradename: Millipore

Eigenschaften

• Produktname: N2A Psen1/Psen2 Double Knockout Mouse Neuroblastoma Cell Line,
• biological source: mouse
• packaging: vial of >1X10⁶ cells
• manufacturer/tradename: Millipore
• growth mode: N/A
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

General description

Early-onset Alzheimer s disease (AD), also termed autosomal dominant AD with early onset (ADAD), is a devastating neurodegenerative condition that is strongly linked to familial inheritance. The main cause of early-onset/familial AD is autosomal dominant

Application

• Each vial contains >1X10⁶ viable cells.

Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
Cells are verified to be of mouse origin and negative for inter-species contamination from rat, Chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
Cells are negative for mycoplasma contamination.

The N2A Psen1/Psen2 cell line is a model for studies of presenilin function in the pathology of familial Alzheimer′s Disease. Early-onset Alzheimer′s disease (AD), also termed autosomal dominant AD with early onset (ADAD), is a devastating neurodegenerative disease that is strongly linked to familial inheritance. The main cause of early-onset/familial AD is autosomal dominant mutations in APP (amyloid precursor protein), PSEN1 (presenilin-1) or PSEN2 (presenilin-2).1 As catalytic subunits of γ-secretase complex, which cleaves APP to form amyloid beta peptides, Psen1 and Psen2 play significant roles in the formation of amyloid plaques. Variations in PSEN1/2 are frequently present in familial early-onset AD cases but their functional significance is unknown. Unraveling the functions of presenilin proteins is thus key to understanding the molecular pathology of AD.

Source
The N2A Psen1/2 cell line was derived via CRISPR/Cas9-editing of the parental N2A mouse neuroblastoma cell line.2 The N2A (Neuro2a) parental cell line was derived from neuroblastoma stem cells of a male strain A/J mouse.4

References
1. Holtzman DM, Morris JC. Goate AM. Sci Transl Med 2011; 3(77): 77sr1.
2. Pimenova AA, Goate AM. Neurobio Dis 2020; 138:104785.
3. Lee E-S, Jeong S-J, Kim Y-H, Jeon C-J. Acta Histochem Cytochem 2015; 48(6): 205-214.
4. Olmsted JB, Carlson K, Klebe R, Ruddle F, Rosenbaum J. Proc Natl Acad Sci USA 1970; 65(1): 129-136.

Features and Benefits

The N2A Psen1/2 cell line is a unique tool for the assessment of the contribution of PSEN mutations to AD pathology and widens the path to targeted therapies for AD and related neurodegenerative diseases.

Preparation Note

Cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Other Notes

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact [email protected].

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable