S-004-M

Enzyme Free Cell Dissociation Solution Hank′s Based (1X), liquid

The Enzyme-Free Cell Dissociation Solution is a Hank′s based formulation of chelating agents & agents to stabilize their activity on cells. This solution is available in a 500ml format.

• Synonym(e): Cell dissociation buffer
• UNSPSC Code: 12352204
• NACRES: NA.75
• eCl@ss: 32160801

Eigenschaften

• Quality Segment: 200
• form: liquid
• manufacturer/tradename: Specialty Media
• technique(s): cell culture | mammalian: suitable, single cell analysis: suitable
• input: sample type: mouse embryonic stem cell(s); sample type neural stem cell(s); sample type mesenchymal stem cell(s); sample type: human embryonic stem cell(s); sample type pancreatic stem cell(s); sample type induced pluripotent stem cell(s); sample type hematopoietic stem cell(s); sample type cardiac stem cell(s); sample type epithelial cells

Beschreibung

General description

Enzyme-Free Cell Dissociation Solution is a Hank′s based formulation of chelating agents and agents to stabilize their activity on cells. This reagent does NOT contain porcine trypsin or any other protein. It works significantly faster than 0.5mM EDTA. In fact, it works as quickly as trypsin on most cells. Most importantly, it preserves the structural and functional integrity of cell surface proteins and does not have the cytotoxic effects sometimes associated with 0.5 mM EDTA. It can be used to dissociate primary cells, tissues, and tumors with an increased plating efficiency.



Enzyme-Free dissociation solution should be used with cells that are sub-confluent (60-80%). If cells are more than 80% confluent, we recommend trypsinizing the cells and replating at 30-40% confluency 24 hours before enzyme-free use (this will allow the cells to be 60-80% confluent for enzyme free dissociation).

Preparation Note

Product can be stored at room temperature or at 4°C. Shelf life is 2 years.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.