The dissection requirement is that the head, liver, and visceral tissues be taken. Unfortunately, other than the source of the dissected tissues, the specific origin is not reported.
PMEF-HL
EmbryoMax® Primary Mouse Embryonic Fibroblasts
Mouse
Synonym(e):
Hygro MEF Feeder Cells, Hygro Mouse Feeder Cells, Hygro-MEFs, Hygromycin Restistant PMEFs, MEF Feeder Cells, hygro PMEFs, hygro MEFs, MEFs, Mouse Feeder Cells
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|
Über diesen Artikel
UNSPSC Code:
41106509
NACRES:
NA.75
eCl@ss:
32011203
Biological source:
mouse
Manufacturer/tradename:
Specialty Media, EmbryoMax®
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenProduktname
EmbryoMax® Primary Mouse Embryonic Fibroblasts, PMEF, Hygro Resistant, Strain C57/BL6, Not Treated, Passage 3
biological source
mouse
Quality Level
manufacturer/tradename
Specialty Media, EmbryoMax®
technique(s)
cell culture | stem cell: suitable
input
sample type: mouse embryonic stem cell(s)
sample type primary embryotic fibroblasts (PMEFs)
sample type induced pluripotent stem cell(s)
shipped in
liquid nitrogen
storage temp.
-140 to -196°C
General description
Primary Mouse Embryo Fibroblasts, hygromycin resistant, strain C57/BL6, are not Mytomycin C treated and serve as a feeder layer for both mouse and human ES/IPS cells. MEF cells are resistant to hygromycin. The cells are provided at passage 3 and can be further expanded before freezing for later use.
Plating MEF Feeder Cells
Procedure:
1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.
Plating MEF Feeder Cells
Procedure:
1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.
The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
Biochem/physiol Actions
Primary Mouse Embryo Fibroblasts
Packaging
5-6x106 ea
Physical form
The cells are provided frozen into individual vials, containing approximately 5-6 x 106 fibroblasts per vial, and are sold as a pack of 5 vials and supplied at passage 3.
Preparation Note
After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
Legal Information
EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany
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Dieser Artikel | |||
|---|---|---|---|
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| technique(s) cell culture | stem cell: suitable | technique(s) cell culture | stem cell: suitable | technique(s) cell culture | stem cell: suitable | technique(s) cell culture | stem cell: suitable |
| shipped in liquid nitrogen | shipped in liquid nitrogen | shipped in liquid nitrogen | shipped in liquid nitrogen |
| storage temp. -140 to -196°C | storage temp. -140 to -196°C | storage temp. -140 to -196°C | storage temp. -140 to -196°C |
| manufacturer/tradename Specialty Media, EmbryoMax® | manufacturer/tradename Specialty Media, EmbryoMax® | manufacturer/tradename Specialty Media, EmbryoMax® | manufacturer/tradename Specialty Media, EmbryoMax® |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Protokolle
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.
Artikel
Mouse embryonic fibroblasts (MEFs) serve as a feeder layer for both mouse and human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPSCs).
Verwandter Inhalt
The cell culture protocols described in this instructional manual include the in vitro culture of murine ES cells using EmbryoMax products along with ESGRO® mLIF medium supplement, as well as feeder-free and serum-free ES cell culture using the ESGRO Complete™ line of products. Also included in this instructional manual are: methods for serum-free neuronal differentiation of mouse ES cells, iPS cell generation using STEMCCA™ lentivirus kits, cre-recombinase mediated excision of STEMCCA™ genes from iPS cells, derivation and rescue of new and existing ES cell lines using RESGRO™ Culture Medium and ESGRO Complete™ system.
"As transgenic mouse core laboratories know well, mouse models are a powerful means of determining the function of mammalian genes in the context of whole organisms—especially since over 95% of the mouse genome is similar to the human genome. However, you may not know that: 1. Laboratory mouse strains are descended from ancestors in modern-day Pakistan. 2. The first inbred strain of lab mouse was created in 1909. It was named DBA for its coat color: dilute brown non-agouti. 3. There is a mutant mouse actually named“Bad Hair Day” (Bhrd). Someone get this little guy a stylist! 4. Male mice, when treated with anticancer drugs, have been shown to pass on DNA instability to their offspring. 5. The founder of Jackson Laboratories, C. C. Little, once had lunch with Walt Disney to request that an animated film be created linking Mickey Mouse and the laboratory mouse. The request was denied."
Global Trade Item Number
| SKU | GTIN |
|---|---|
| PMEF-HL | 04053252615238 |
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Would it be possible to get a more precise information on the dissection of these fibroblasts -- i.e. were the head, vertebral column, dorsal root ganglia, and visceral organs removed? Or were these fibroblasts obtained from limbs only?
1 answer-
Helpful?
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