OB05
Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2)
liquid, clone 13/45/31/2, Calbiochem®
Synonym(e):
Anti-His6
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About This Item
UNSPSC-Code:
12352203
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified antibody
Antikörper-Produkttyp
primary antibodies
Klon
13/45/31/2, monoclonal
Form
liquid
Enthält
≤0.1% sodium azide as preservative
Speziesreaktivität (Voraussage durch Homologie)
all
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
do not freeze
Isotyp
IgG1
Versandbedingung
wet ice
Lagertemp.
2-8°C
Posttranslationale Modifikation Target
unmodified
Allgemeine Beschreibung
Anti-Histidine-Tagged Protein, mouse monoclonal, clone 13/45/31/2, specifically recognizes an epitage of 6 consecutive His residues. It is validated for use in ELISA, WB, IF & IP.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes a His•Tag sequence in native and recombinant proteins. Specifically recognizes an epitope of six consecutive His residues of both natural and recombinant sources.
Specifically recognizes an epitage of six consecutive His residues of both natural and recombinant sources.
Immunogen
Epitope: HHHHHH
a recombinant protein containing a His•Tag sequence
Anwendung
ELISA (0.1-0.4 g/ml)
Immunoblotting (1-4 g/ml)
Immunofluorescence (1-4 g/ml)
Immunoprecipitation (4 g/ml)
Verpackung
Please refer to vial label for lot-specific concentration.
Warnhinweis
Toxicity: Standard Handling (A)
Physikalische Form
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Hinweis zur Analyse
Positive Control
Proteins containing a His•Tag sequence
Proteins containing a His•Tag sequence
Sonstige Hinweise
Antibody should be titrated for optimal results in individual systems.
Zentgraf, H., et al. 1995. Nucl. Acids. Res.23, 3347.
Gu, J., et al. 1994. BioTechniques17, 257.
Sporeno, E., et al. 1994. J. Biol. Chem., 10991.
Sisk, W.P., et al. 1994. J. Virol.68, 766.
Lu, T., et al. 1993. Anal. Biochem.213, 318.
Garner, J., et al. 1992. Cell69, 833.
Hochuli, E., et al. 1987. J. Chromatogr.411, 177.
Gu, J., et al. 1994. BioTechniques17, 257.
Sporeno, E., et al. 1994. J. Biol. Chem., 10991.
Sisk, W.P., et al. 1994. J. Virol.68, 766.
Lu, T., et al. 1993. Anal. Biochem.213, 318.
Garner, J., et al. 1992. Cell69, 833.
Hochuli, E., et al. 1987. J. Chromatogr.411, 177.
Rechtliche Hinweise
Manufactured by Dianova, GmbH, Germany.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Maureen C Dolan et al.
Methods in molecular biology (Clifton, N.J.), 824, 65-105 (2011-12-14)
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Samadhan J Jadhao et al.
Journal of medical virology, 93(6), 3439-3445 (2020-12-17)
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