MABT29

Anti-Ninein Antibody, clone 79-160-7

clone 79-160-7, from mouse

• Synonym(e): ninein (GSK3B interacting protein), Glycogen synthase kinase 3 beta-interacting protein, ninein centrosomal protein, GSK3B-interacting protein
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 79-160-7, monoclonal
• Application: IP, WB
• Citations: 10

Eigenschaften

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 79-160-7, monoclonal
• species reactivity: human
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_891989
• UniProt accession no.: Q8N4C6
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... NIN(51199)

Beschreibung

General description

Ninein is a coiled-coil, centrosomal protein that is a necessary component in microtubule minus-end anchorage and positioning, and may have a role as a maturation factor for centrosomes. It may also be involved in microtubule nucleation. More recent studies have identified ninein as a crucial component in the formation of blood vessels. Expression of ninein is ubiquitous, but significantly higher levels have been observed in skeletal muscle and heart tissue. There have been 8 isoforms characterized due to alternative splicing.

~ 250 kDa observed MW. An unidentified nonspecific band appears at ~ 50 kDa

Immunogen

Recombinant protein corresponding to human Ninein.

Application

Anti-Ninein Antibody, clone 79-160-7 is an antibody against Ninein for use in WB & IP.

Immunocytochemistry Analysis: 1:500 dilution of the antibody has been shown to detect Ninein in HeLa and A431 cells.

Immunoprecipitation Analysis: The antibody has been shown to immunoprecipitate Ninein in L929 cells. (Delgehyr, 2005).

Physical form

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.

Analysis Note

Evaluated by Western Blot in Hek293 cell lysate.

Western Blot Analysis: 1 µg/mL of the antibody detected Ninein in 10 µg of Hek293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable