MABS409

Anti-NFATc1 Antibody, clone 7A6

clone 7A6, from mouse

• Synonym(e): Nuclear factor of activated T-cells, cytoplasmic 1, NF-ATc1, NFATc1, NFAT transcription complex cytosolic component, NF-Atc, NFATc
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 7A6, monoclonal
• Species reactivity: human, mouse
• Application: FACS, IF, WB
• Citations: 6

Eigenschaften

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 7A6, monoclonal
• species reactivity: human, mouse
• technique(s): flow cytometry: suitable, immunofluorescence: suitable, western blot: suitable
• isotype: IgG1
• UniProt accession no.: O95644
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... NFATC1(4772)

Beschreibung

General description

NFATc1, also known as Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1, is a component of the nuclear factor of activated T cells DNA-binding transcription complex which consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. This protein plays a main role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription and functions as a major molecular target for the immunosuppressive drugs such as cyclosporine A. NFATc1 is expressed in most human primary lymphocytes and mature human T- and B-cell neoplasms.

~~95, ~110 and ~130 kDa observed

Immunogen

Recombinant protein of human NFATc1 amino acids 197-304

Application

Detect NFAT using this mouse monoclonal antibody, Anti-NFATc1 Antibody, clone 7A6 validated for use in western blotting, Flow Cytometry & Immunofluorescence.

Flow Cytometry Analysis: A representative lot detected human NFATc1 in Flow Cytometry. (Brandt C, et al. 2010.)

Immunofluorescent Analysis: A representative lot detected human NFATc1 in Immunofluorescence. (Timmerman LA, et al. 1997.)

Physical form

Format: Purified

Purified mouse monoclonal IgG1 antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide.

Preparation Note

The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Analysis Note

Evaluated by Western Blotting in Jurkat, human Th1, and mouse Th1 cell extracts.

Western Blotting Analysis: 1 μg/mL of this antibody detected in Jurkat, human Th1, and mouse Th1 cell extracts.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable