MABN40

Anti-LRRK2 Antibody, clone N138/6

clone N138/6, from mouse

• Synonym(e): leucine-rich repeat kinase 2, DARDARIN, augmented in rheumatoid arthritis 17, Parkinson disease (autosomal dominant) 8, Dardarin
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: N138/6, monoclonal
• Application: IHC, WB
• Citations: 2

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: N138/6, monoclonal
• species reactivity: human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_940980
• UniProt accession no.: Q5S007
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... LRRK2(120892)

Beschreibung

General description

Leucine-rich repeat kinase 2 (LRRK2) is a 2,527 amino acid protein encoded by the PARK8 gene and belonging to the ROCO family of proteins. This protein family is characterized as having a unique interaction between the Roc domain and the intrinsic GTPase activity along with a COR domain. LRKK2 consists of 5 different domains; a tyrosine kinase-like MAPKKK-related domain, WD40 propeller motif repeats, a Roc (Ras-like small GTPase family) domain, a COR (C-terminal of Roc) domain, and an LRRK. LRRK2 is mainly localized to the cytoplasm and expression within tissue is most highly observed within the brain. It is found in lower levels within the liver, heart, lung, skeletal muscles, placenta, pancreas, and kidney. The exact function of LRRK2 is unknown; however mutations to any of the five domains are the most commonly known causes of Parkinson’s disease, both familial and sporadic. Further study of this protein may lead to a better understanding of the disease’s pathogenesis, as well as potential therapeutic markers.

240 kDa observed. 286 kDa calculated. (Glasson, B., et al. (2006). Ann. Neurol. 59(2):315-322.)

Immunogen

Recombinant protein corresponding to human LRRK2.

Application

Anti-LRRK2 Antibody, clone N138/6 detects level of LRRK2 & has been published & validated for use in IH & WB.

Western Blot Analysis: A previous lot was used by an independent laboratory in WB. (UC Davis/NIH NeuroMab Facility, www.neuromab.org, [email protected])

Biochem/physiol Actions

This antibody recognizes LRRK2.

Physical form

Format: Purified

Analysis Note

Control
Human placenta and Parkinson′s diseased vascular brain tissue

Evaluated by Immunohistochemistry in human placenta and Parkinson′s diseased vascular brain tissue.

Immunohistochemistry Analysis: 1:300 dilution of this antibody detected LRRK2 on 10 µg of human placenta and Parkinson′s diseased vascular brain tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable