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MABC1193

Sigma-Aldrich

Anti-LC3A/B Antibody, clone 4F11.1

clone 4F11.1, from mouse

Synonym(e):

Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 li

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

4F11.1, monoclonal

Speziesreaktivität

human

Verpackung

antibody small pack of 25 μg

Methode(n)

western blot: suitable

Isotyp

IgG2aκ

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

ambient

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

Allgemeine Beschreibung

Microtubule-associated proteins 1A/1B light chain 3B (UniProt: Q9GZQ8; also known as Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta) is encoded by the MAP1LC3B (also known as MAP1ALC3) gene (Gene ID: 81631) in human. Autophagy-related protein LC3 A/B is an ubiquitin-like modifier that is involved in formation of autophagosomal vacuoles (autophagosomes). It plays a role in mitophagy that contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess reactive oxygen species production. It contains a microtubule-associated proteins 1A/1B light chain 3B (aa 2-120) and a propeptide region (aa 121-125) that is cleaved off in mature form. The precursor molecule is cleaved by ATG4B (between aa 120-121) to form the cytosolic form LC3-I, which is activated by ATG7 and then transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) to generate the membrane-bound LC3-II. Phosphorylation at threonine12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Autophagy-related protein LC3 B is found to be most abundant in heart, brain, skeletal muscle and testis.

Spezifität

Clone 4F11.1 detects Autophagy-related protein LC3 B in human cells.

Immunogen

GST/His-tagged recombinant fragment corresponding to amino acids 2-74 of human Autophagy-related protein LC3 B.

Anwendung

Research Category
Apoptose & Krebs
Anti-LC3A/B, clone 4F11.1, Cat. No. MABC1193, is a mouse monoclonal antibody that detects LC3A/B and has been tested for use in Western Blotting.

Qualität

Evaluated by Western Blotting in HeLa cells treated with 50 µM chloroquine.

Western Blotting Analysis: 1 µg/mL of this antibody detected LC3A/B in 10 µg of lysate from HeLa cells treated with 50 µM chloroquine.

Zielbeschreibung

~15 kDa observed; 14.69 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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