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MABC004F

Sigma-Aldrich

Mouse IgG2a Negative Control, clone GC270, FITC conjugate

Mouse IgG2a Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.42

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

FITC conjugate

Antikörperform

ascites fluid

Klon

GC270, monoclonal

Hersteller/Markenname

Chemicon®

Methode(n)

flow cytometry: suitable
immunofluorescence: suitable

Isotyp

IgG2a

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Spezifität

This antibody reacts with the outer membrane protein of some strains of Neisseria gonorrhoeae. No reaction with human cell surface or plasma components has been observed. However, some binding to Fc receptors may occur.

Immunogen

Protein I preparation from Neisseria gonorrhoeae.

Anwendung

Research Category
Sekundär- & Kontrollantikörper

Epitop-Tags & allgemeine Anwendung
Research Sub Category
Isotypspezifische Kontrollantikörper
Fluorescein conjugated mouse IgG2a is a monoclonal antibody intended for use as a negative control in immunofluorescence, flow cytometry. Its use enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.

SUGGESTED USAGE

Flow cytometry and immunofluorescence - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) or bone marrow cells in 100 μl buffer.
Mouse IgG2a Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

Physikalische Form

Purified from mouse ascitic fluid. Fluorescein conjugated, ready to use. The antibody is supplied in 1.0ml phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.

Lagerung und Haltbarkeit

Store at 2 to 8°C, for up to 6 months. DO NOT FREEZE. Protect from light.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Huifen Li et al.
Journal of virological methods, 221, 127-130 (2015-05-11)
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