MAB4140

Anti-MDR3 Antibody, clone P3 II-26

culture supernatant, clone P3 II-26, Chemicon^®

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: P3 II-26, monoclonal
• Species reactivity: human
• Application: ICC, IHC, WB
• Citations: 9

Eigenschaften

• biological source: mouse
• Quality Segment: 300
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: P3 II-26, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG2b
• suitability: not suitable for immunohistochemistry (Paraffin)
• NCBI accession no.: NM_000443.3, NM_018849.2, NM_018850.2
• UniProt accession no.: P21439
• shipped in: wet ice
• Gene Information: human ... ABCB4(5244)

Beschreibung

Immunogen

Mab was selected after immunization with a fusion protein consisting of Gluthatione-S-Transferase and a fragment of MDR3 P-gp comprising aa 629-692.

Application

Anti-MDR3 Antibody, clone P3 II-26 is an antibody against MDR3 for use in WB, IC, IH.

Western Blotting: MDR3 is a 140 kDa protein.

Immunocytochemistry: 1:20 - 1:50 on acetone-fixed cytospin preparations

Immunohistochemistry: 1:20 on acetone-fixed frozen sections

Not suitable for paraffin-embedded tissues

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

This antibody reacts with an internal epitope of MDR3 P-gp. It does not cross-react with the human MDR1 P-gp. It has been reported that this clone also cross reacts with mouse MDR2 Pgp {Kok et al 2003 {http://www.biochemj.org/bj/369/0539/3690539.pdf}.

Preparation Note

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 2