MAB3750

Anti-LEF-1 Antibody, all isoforms, clone 1C3.1D10

clone 1C3.1D10, Chemicon^®, from mouse

• Synonym(e): Lymphoid Enhancer Factor 1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 1C3.1D10, monoclonal
• Speziesreaktivität: human
• Hersteller/Markenname: Chemicon^®
• Methode(n): western blot: suitable
• Aufnahme: sample type hematopoietic stem cell(s)
• Isotyp: IgG1
• NCBI-Hinterlegungsnummer: NM_016269.2
• UniProt-Hinterlegungsnummer: Q9UJU2
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... LEF1(51176)

Beschreibung

Allgemeine Beschreibung

Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 256-308 of LEF-1. This region is between the transactivation domain and the HMG binding domain of LEF-1 and is conserved in all LEF-1 isoforms.

Spezifität

Recognizes human LEF-1 (Lymphoid Enhancer Factor-1) protein. Does not cross-react with TCF-1 or TCF-4.

Immunogen

Epitope: all isoforms

Recombinant full length LEF-1 protein.

Anwendung

Research Category
Stammzellforschung

Research Sub Category
Neurale Stammzellen

Hämatopoetische Stammzellen

Anti-LEF-1 Antibody, all isoforms, clone 1C3.1D10 is an antibody against LEF-1 for use in WB.

Western blot

Optimal working dilutions must be determined by end user.

Verlinkung

Replaces: 04-1130

Physikalische Form

Format: Purified

Purified immunoglobulin. Provided as solution in phosphate buffered saline with 0.08% sodium azide.

Lagerung und Haltbarkeit

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Hinweis zur Analyse

Control
Jurkat cell lysate and human colon adenocarcinoma tissue

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable