MAB3222

Anti-BrdU Antibody, clone IIB5

clone IIB5, Chemicon^®, from mouse

• Synonym(e): BrdU
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: IIB5, monoclonal
• Technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin)
• Application: FACS, ICC, IHC
• Citations: 17

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: IIB5, monoclonal
• species reactivity (predicted by homology): all
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin)
• isotype: IgG1
• shipped in: wet ice
• target post-translational modification: unmodified

Beschreibung

General description

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Immunogen

A fusion of mouse myeloma cells with spleen cells from a mouse intraperitoneally immunized with BrdU conjugated to bovine serum albumin.

Application

Anti-BrdU Antibody, clone IIB5 is a Mouse Monoclonal Antibody for detection of Bromodeoxyuridine also known as BrdU & has been validated in FC, ICC, IHC(P).

Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissues with acid/proteinase treatments. See:

http://www.protocol-online.org/prot/Immunology/Immunohistochemistry. For examples of various protocols.

Flow Cytometry: 1:100-1:200; acid methanol treatment required.

Optimal working dilutions must be determined by the end user.

In order for the antibody to work a pretreatment to expose DNA will be needed (1 mg/mL of pepsin in 0.01 M HCL 30 min at r/t)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Biochem/physiol Actions

Reacts specifically with bromodeoxyuridine. It also recognizes BrdU when incorporated into nuclear DNA.

Physical form

Format: Purified

Protein A Purified mouse immunoglobulin in PBS with 0.09% sodium azide.

Protein A purified

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
After incorporation of BrdU, all DNA containing species

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable