CP18

Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11)

liquid, clone II-4C11, Calbiochem^®

• Synonym(e): Collagen Type II antibody
• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: II-4C11, monoclonal
• Species reactivity: rat, human, bovine, rabbit
• Application: —
• Citations: 42

Eigenschaften

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: II-4C11, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: rat, human, bovine, rabbit
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (Frozen Sections (1-10 µg/mL); Immunoblotting (1-5 µg/mL, ; Paraffin Sections (1-10 µg/mL, )
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... COL2A1(1280)

Beschreibung

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-X-63-Ag8-LUI mouse myeloma cells. Recognizes the ~130 kDa type II collagen protein.

Recognizes the ~130 kDa type II collagen protein. May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected.

This Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11) is validated for use in Frozen Sections for the detection of Collagen Type II (Ab-1).

Immunogen

Human

purified collagen type II

Application

Frozen Sections (1-10 µg/ml)

Immunoblotting (1-5 µg/ml, see application references)

Paraffin Sections (1-10 µg/ml, see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Kumagai, J. et al. 1994 J. Anat.185, 278.
Kivrikko, K,I. 1993 Ann. Med.25, 113.
Sandell, L.J., et al. 1991 J. Cell. Biol.114, 1307.
Ryan, M.C., and Sandell, L.J. 1990 J. Biol. Chem.265, 10334.
Mayne, R., and Burgeson, R.E. 1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL

May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected. This antibody may also react with other classes of collagen at a concentration higher than 1 µg/ml. In immunoblotting, a major band of ~130 kDa is obtained. Antibody should be titrated for optimal results in individual systems.

Legal Information

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable