APT131
Caspase 3 Colorimetric Activity Assay Kit, DEVD
Caspase-3 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence DEVD.
Synonym(e):
CPP32
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Über diesen Artikel
UNSPSC Code:
12161503
NACRES:
NA.54
eCl@ss:
32161000
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenQuality Level
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Chemicon®
technique(s)
activity assay: suitable
NCBI accession no.
UniProt accession no.
detection method
colorimetric
shipped in
dry ice
Gene Information
human ... CASP3(836)
General description
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon′s Caspase-3 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity.
Application
Caspase-3 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence DEVD.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Preparation Note
Store kit materials at -20°C up to their expiration date.
Special Precautions:
· After thawing reagents, use immediately or aliquot and freeze at -20°C for longer storage. Avoid repeated freeze/thaw cycles.
· The Caspase-3 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
Special Precautions:
· After thawing reagents, use immediately or aliquot and freeze at -20°C for longer storage. Avoid repeated freeze/thaw cycles.
· The Caspase-3 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.
Other Notes
5X Cell Lysis Buffer (Part No. 90065): 5 mL
5X Assay Buffer (Part No. 90066): 10 mL
Caspase-3 Substrate (Ac-DEVD-pNA) (Part No. 90079): 250 μL of 3 mg/mL solution
Caspase-3 Inhibitor (Ac-DEVD-CHO) (Part No. 90067): 50 μL of 100 μM (0.05 mg/mL) in DMSO
pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
5X Assay Buffer (Part No. 90066): 10 mL
Caspase-3 Substrate (Ac-DEVD-pNA) (Part No. 90079): 250 μL of 3 mg/mL solution
Caspase-3 Inhibitor (Ac-DEVD-CHO) (Part No. 90067): 50 μL of 100 μM (0.05 mg/mL) in DMSO
pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Dieser Artikel | |||
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| Gene Information human ... CASP3(836) | Gene Information human ... CASP3(836) | Gene Information human ... CASP3(836) | Gene Information human ... CASP3(836) |
| species reactivity (predicted by homology) mammals | species reactivity (predicted by homology) mammals | species reactivity (predicted by homology) - | species reactivity (predicted by homology) mammals |
| Quality Level 100 | Quality Level 100 | Quality Level 300 | Quality Level 100 |
| manufacturer/tradename Chemicon® | manufacturer/tradename Chemicon® | manufacturer/tradename - | manufacturer/tradename CaspaTag, Chemicon® |
| technique(s) activity assay: suitable | technique(s) activity assay: suitable | technique(s) - | technique(s) activity assay: suitable, flow cytometry: suitable, immunofluorescence: suitable |
| detection method colorimetric | detection method colorimetric | detection method - | detection method fluorometric |
Lagerklasse
10 - Combustible liquids
wgk
WGK 2
Analysenzertifikate (COA)
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M Uzan et al.
Acta neurochirurgica, 148(11), 1157-1164 (2006-09-12)
To determine whether sFas, caspase-3, proteins which propagate apoptosis, and bcl-2, a protein which inhibits apoptosis, would be increased in cerebrospinal fluid (CSF) in patients with severe traumatic brain injury (TBI) and to examine the correlation of sFas, caspase-3, and
Giuseppe M Campo et al.
Journal of applied toxicology : JAT, 28(4), 509-517 (2007-09-20)
Oxidative stress, inflammation and apoptosis play a critical role in the onset and progression of cellular damage. It was previously reported that hyaluronan (HA) and chondroitin-4-sulphate (C4S) were able to protect human skin fibroblasts from oxidative stress. This antioxidant activity
Giuseppe M Campo et al.
Molecular and cellular biochemistry, 311(1-2), 167-177 (2008-01-29)
Previous studies suggest that Transforming growth factor-1beta (TGF-1beta) administration in human fibroblasts exposed to oxidative stress is able to modulate hyaluronan synthases (HASs). HAS modulation in turn increases high molecular weight (Hyaluronan) HA concentration. Nuclear factor kB (NF-kB) is a
Global Trade Item Number
| SKU | GTIN |
|---|---|
| APT131 | 04053252515293 |