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ACK5003NT

Amicon® Pro Affinity Concentration Kit Ni-NTA with 3kDa Amicon® Ultra-0.5 Device

Amicon Pro Affinity Concentration Kit Ni-NTA - A Centrifugal Tool for purifying & concentrating His-tagged proteins.

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Über diesen Artikel

eCl@ss:
36100101
UNSPSC Code:
41104916
Material:
Amicon Ultra styrene-butadiene device, Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding), polyethylene cap, polyethylene frit, polypropylene device (for exchange), polypropylene frit, polypropylene tube (AU0.5; for collection), polypropylene tube (for collection), polypropylene tube (holder)
Pore size:
3 kDa NMWCO
Working volume:
10 mL
Sterility:
non-sterile


material

Amicon Ultra styrene-butadiene device, Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding), polyethylene cap, polyethylene frit, polypropylene device (for exchange), polypropylene frit, polypropylene tube (AU0.5; for collection), polypropylene tube (for collection), polypropylene tube (holder)

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Pro

parameter

10 mL sample volume

technique(s)

buffer exchange: suitable, protein purification: suitable (antibody labeling), protein purification: suitable (concentration)

L

12 cm (4.7 in.)

diam.

2.97 cm

filtration area

1 cm2

working volume

10 mL

pore size

3 kDa NMWCO

shipped in

wet ice

General description

Filter Code: PLCGC
Filter Type: Ultrafiltration
This kit includes 12/pk Amicon Pro, 12/pk Amicon Ultra NMWCO 3 kDa, 3 ml HisBind Ni-NTA resin (Binding capacity: 5 to 10mg His tagged protein/ml agarose), 25ml Bind Buffer (4X), 25 ml Wash buffer (4X), 5ml elution buffer (4X)

Application

Amicon Pro Affinity Concentration Kit Ni-NTA - A Centrifugal Tool for purifying & concentrating His-tagged proteins.

Features and Benefits

Minimum Final Concentrate Volume: 15 µL

Physical form

Color Code: Clear with pink cap

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany


signalword

Danger

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 3 - Repr. 1B - Skin Corr. 1C

Lagerklasse

3 - Flammable liquids

flash_point_f

95.9 °F

flash_point_c

35.5 °C



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Verwandter Inhalt

When you start with consistent yields of active, native-folded protein, you’re giving your experiment the best chance to succeed. If your current protein preps involve juggling columns, dialyzers and multiple transfer steps, you could be introducing variability into your data. For your next protein preparation, choose the simple, gentle method that tackles even the most labile and poorly expressed proteins—the Amicon® Pro purification system. When your proteins behave, your research will flourish.

Two attributes of the Amicon® Pro device make it a convenient device for preparing pure, labeled antibody. First, the device enables highly efficient buffer exchange via diafiltration with simultaneous sample concentration in a single 15 minute spin. Second, the entire workflow can be performed within a single device, reducing the potential for sample loss. In this report, we describe the successful use of the Amicon® Pro device for small–scale biotinylation of a target antibody. The biotinylated antibody was used for detection of protein immunoprecipitated from cell lysate.

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Global Trade Item Number

SKUGTIN
ACK5003NT04053252911590

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