ABT142

Anti-PLK-4 Antibody

from rabbit

• Synonym(e): Serine/threonine-protein kinase PLK4, Polo-like kinase 4, PLK-4, Serine/threonine-protein kinase 18, Serine/threonine-protein kinase Sak
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, WB
• Citations: -

Eigenschaften

• Quality Level: 100
• biological source: rabbit
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, rat, human
• technique(s): immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_0055079
• UniProt accession no.: O00444
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PLK4(10733)

Beschreibung

General description

Serine/threonine-protein kinase PLK4 (PLK-4) is a member of the protein kinase superfamily and plays an essential role in centriole duplication. PLK-4 triggers procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins. It may be involved in tumorigenesis since centrosome aberrations are frequently observed in tumors. PLK-4 may also be involved in trophoblast differentiation by phosphorylating HAND1, leading to the disruption between HAND1 and MDFIC and then activating HAND1.

~95 kDa observed

Immunogen

Epitope: POLO box domain

KLH-conjugated linear peptide corresponding to the POLO box domain of human PLK-4.

Application

Anti-PLK-4 Antibody detects level of PLK-4 & has been published & validated for use in Western Blotting, ICC.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Biochem/physiol Actions

This antibody recognizes the POLO box domain of PLK-4

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Jurkat cell lysate

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected PLK-4 in 10 µg of Jurkat cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable