ABE543

Anti-PRDM16 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): PR domain zinc finger protein 16, PR domain-containing protein 16, Transcription factor MEL1, MDS1/EVI1-like gene 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, IP, WB
• Citations: 1

Eigenschaften

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): horse (based on 100% sequence homology), canine (based on 100% sequence homology), bovine (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology), guinea pig (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable ((Paraffin)), immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_071397
• UniProt accession no.: Q9HAZ2
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PRDM16(63976)

Beschreibung

General description

Anti-PRDM16, Cat. No. ABE543, is a rabbit polyclonal antibody that detects PR domain zinc finger protein 16 (PRDM16) and is teste for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Target molecular weight ~140 kDa observed; 140.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

~140 kDa observed. Four isoforms are known to exist. This protein has been observed at 150 kDa, 135 kDa, and 100 kDa (EMD Millipore Cat. No. ABD130). Uncharacterized band(s) may be observed in some cell lysates.

Immunogen

KLH-conjugated linear peptide corresponding to human PRDM16.

Application

Detect PRDM16 using this rabbit polyclonal antibody, Anti-PRDM16 Antibody validated for use in western blotting, IP & IHC.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated PRDM16 from 500 µg of K562 nuclear extract.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected PRDM16 in human kidney tissues.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Physical form

Affinity purified

Purified rabbit serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in LNCaP nuclear extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected PRDM16 in 10 µg of LNCaP nuclear extract.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable