AB5350

Anti-Potassium Channel SK3 Antibody

Chemicon^®, from rabbit

• Synonym(e): KCa3, Kcnn3, SKCa3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 4

Eigenschaften

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, rat
• manufacturer/tradename: Chemicon^®
• technique(s): western blot: suitable
• NCBI accession no.: NM_002249.4, NM_170782.1
• UniProt accession no.: Q9UGI6
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KCNN4(3783)

Beschreibung

Immunogen

Highly purified peptide corresponding to residues 2-21 of human SK3 (Accession Q9UGI6).

Application

Anti-Potassium Channel SK3 Antibody detects level of Potassium Channel SK3 & has been published & validated for use in WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blotting: 1.0-1.5 μg/mL (1:200-1:300)

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes SK3. The epitope specific for SK3 is not present in any other known proteins. The immunogen sequence is identical in mouse and porcine.

Physical form

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.025% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Preparation Note

Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of sterile distilled water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3