AB3579

Anti-BimEL (pS69) human / (pS65) rat Antibody

Chemicon^®, from rabbit

• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: rat, human
• Speziesreaktivität (Voraussage durch Homologie): mouse (100% homologous)
• Hersteller/Markenname: Chemicon^®
• Methode(n): western blot: suitable
• NCBI-Hinterlegungsnummer: NM_006538.3; NM_138621.3; NM_138622.2; NM_138623.2; NM_138624.2; NM_138626.2; NM_138627.2; NM_207002.2; NM_207003.1
• UniProt-Hinterlegungsnummer: O43521
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: phosphorylation (pSer69)
• Angaben zum Gen: human ... BCL2L11(10018)

Beschreibung

Allgemeine Beschreibung

Bim (bcl-2-interacting mediator of cell death) is a proapoptotic member of the Bcl-2 family that shares only the BH3 domain with this family. There are three isoforms of Bim: Bim_EL, Bim_L, and Bim_S. Bim is involved in regulating the intrinsic mitochondrial apoptotic pathway by inducing cytochrome c release, which in turn, activates caspase-9 and then caspase-3. Bim also plays a critical role in central and peripheral deletion of T lymphocytes and in controlling B cell homeostasis and activation. Bim_EL, the long isoform of Bim, is a ~28 kDa protein that is predominantly expressed in T and B cells and is activated by ERK1/2 pathway. The activation of Bim_EL by ERK1/2 promotes its phosphorylation on serine 65 (serine 69 in human), targeting it for degradation via the proteasome.

Spezifität

Rat Bim_EL when phosphorylated at serine 65. Human Bim_EL when phosphorylated at serine 69. Mouse Bim_EL (100% conserved) has not been tested, but is expected to react.

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of rat Bim_EL that contains serine 65 (serine 69 in the human sequence). The sequence is conserved in human and mouse.

Anwendung

Research Category
Apoptose & Krebs

Research Sub Category
BCL2 & Hemmung

Anti-BimEL (pS69) human / (pS65) rat Antibody is an antibody against BimEL (pS69) human / (pS65) rat for use in WB.

For Western blotting applications, we recommend using the antibody at a 1:1000 dilution.

Qualität

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bim. The final product is generated by affinity chromatography using a Bim_EL-derived peptide that is phosphorylated at serine 65 (serine 69 in the human sequence).

Physikalische Form

Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide

Lagerung und Haltbarkeit

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.

Hinweis zur Analyse

Control
Hek293T co-expressing rat Bim_EL and active MEKK1 (MEKK1δ).

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2