After verification, an anti-doublecortin antibody recommended for free-floating application on mouse brain tissue is not currently available. However, a citation on AB2253 for free-floating application on mouse brain tissue was found and may be of interest for reference.
Product: AB2253 Sigma-Aldrich Anti-Doublecortin Antibody
Publication: Nat Commun. 2015 Mar 26;6:6606
Title: Aberrant hippocampal neurogenesis contributes to epilepsy and associated cognitive decline
PMID: 25808087
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 50 μL | Warenkorb auf Verfügbarkeit prüfen | € 618,00 |
Über diesen Artikel
biological source
guinea pig
Quality Segment
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse
species reactivity (predicted by homology)
human (based on 100% sequence homology)
technique(s)
immunofluorescence: suitable (Frozen tissue sections), immunohistochemistry: suitable (Paraffin), western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... DCDC1(341019)
General description
Immunogen
Application
Biochem/physiol Actions
Physical form
Preparation Note
Analysis Note
Western-Blot-Analyse: Eine 1:1000 Verdünnung dieses Antikörpers diente zum Nachweis von Doublecortin in Rattenhirn-Gewebelysat.
Rattenhirn-Lysat.
Other Notes
Disclaimer
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| biological source guinea pig | biological source rabbit | biological source rabbit | biological source rabbit |
| Quality Level 100 | Quality Level 200 | Quality Level - | Quality Level - |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| antibody form serum | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| technique(s) immunofluorescence: suitable (Frozen tissue sections), western blot: suitable, immunohistochemistry: suitable (Paraffin) | technique(s) western blot: 1-2 μg/mL using HEK-293T cells expressing human doublecortin. Also, mouse and rat brain extract (S1 fraction). | technique(s) ELISA: 1:10000, western blot: 1:500-1:1000 | technique(s) ELISA: 1:40000, western blot: 1:500-1:1000 |
| Gene Information human ... DCDC1(341019) | Gene Information human ... DCX(1641) | Gene Information human ... DCX(1641) | Gene Information human ... PIGX(54965) |
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Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Verwandter Inhalt
Global Trade Item Number
| SKU | GTIN |
|---|---|
| AB2253 | 04053252335495 |
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Is it possible to obtain a small aliquot of specific antibodies for testing purposes to determine their suitability for staining Doublecortin (DCX) in immunohistochemistry on free-floating mouse brain tissue fixed with PFA? The antibodies of interest are the guinea pig polyclonal and the goat polyclonal.
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