72350

BacMagic-3 DNA Kit - Novagen

• Synonym(e): BacMagic DNA, BacMagic DNA Kits
• NACRES: NA.54
• UNSPSC Code: 12352200

Eigenschaften

• manufacturer/tradename: Novagen^®
• Quality Level: 100
• availability: not available in Japan
• storage condition: OK to freeze
• shipped in: wet ice
• storage temp.: 2-8°C

Beschreibung

General description

The BacMagic-3 DNA Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. Derived from an AcNPV genome, BacMagic-3 DNA is deleted for a portion of the essential open reading frame (ORF) 1629, preventing nonrecombinant virus form replicating in insect cells. The nonessential chitinase (chiA) gene is deleted to improve production of secreted and membrane target proteins. Additionally, the v-cath gene, a cathespsin-like cysteine protease, is deleted resulting in significantly improved quality and yield for most target proteins. Unlike BacMagic-2, the BacMagic-3 genome is deleted for an additional three viral genes (p10, p24, and p26). These genes are nonessential to virus in tissue culture and improve protein expression. The BacMagic-3 DNA Kit contains the BacMagic-3 DNA, Insect GeneJuice^® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency.

Other Notes

• 0.5 µg- BacMagic-3 DNA
• 50 µl- Insect GeneJuice Transfection Reagent
• 2 µg- Transfection Control Plasmid

Legal Information

GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• wgk: WGK 2