650211

Trypsin, Excision Grade, Bovine Pancreas

• Synonym(e): Trypsin, Excision Grade, Bovine Pancreas
• CAS-Nummer: 9002-07-7
• UNSPSC Code: 12352202
• EG-Nummer: 3.4.21.4 (BRENDA, IUBMB)

Eigenschaften

• assay: ≥90% (HPLC)
• Quality Segment: 100
• form: solid
• specific activity: ≥80 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated
• color: white
• solubility: trifluoroacetic acid: 0.01%, HCl: 1 mM
• foreign activity: Chymotrypsin ≤0.05%
• shipped in: ambient
• storage temp.: 2-8°C

Beschreibung

General description

Native trypsin from bovine pancreas. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis. Designed for protein sequencing or sequence verification, analysis of protein structural domains and cleavage of fusion proteins.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Stock solutions are stable for up to 1 month at -20°C or for up to 1 week at 4°C.

Other Notes

1 unit is the amount of enzyme that will convert 1 µmol of substrate per min at 37°C in 100 mM Tris pH 8.5

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Sicherheitshinweise

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1