567370

Smac-N7 Peptide

A peptide that contains the N-terminal seven residues of Smac (Second Mitochondria-derived Activator of Caspases, also known as DIABLO) and promotes procaspase-3 activation at around 10 µM.

• Synonym(e): Smac-N7 Peptide, H-AVPIAQK-OH
• Empirische Formel (Hill-System): C₃₃H₅₉N₉O₉
• Molekulargewicht: 725.88
• UNSPSC Code: 12352200
• Assay: ≥95% (HPLC)
• Form: lyophilized solid
• Storage condition: OK to freeze

Eigenschaften

• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: water: 1 mg/mL, DMSO: 5 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Beschreibung

General description

A peptide that contains the N-terminal seven residues of Smac (Second Mitochondria-derived Activator of Caspases, also known as DIABLO) and promotes procaspase-3 activation at around 10 µM. Reported to bind to the BIR2 and BIR3 (baculovirus IAP repeat) domains of XIAP (X-linked inhibitor of apoptosis proteins) with a K_d ~800 nM. Promotes the catalytic activity of caspase-9 in the presence of XIAP, suggesting that Smac competes with caspase-9 for binding to the same pocket on the surface of XIAP.

Biochem/physiol Actions

Cell permeable: no

Kd ~800 nM in binding to the BIR2 and BIR3 (baculovirus IAP repeat) domains of XIAP (X-linked inhibitor of apoptosis proteins)

Primary Target
Promotes the catalytic activity of caspase-9 in the presence of XIAP

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

H-Ala-Val-Pro-Ile-Ala-Gln-Lys-OH

Srinivasula, S.M., et al. 2001. Nature410, 112.
Chai, J., et al. 2000. Nature406, 855.
Liu, Z., et al. 2000. Nature408, 1004.
Srinivasula, S.M., et al. 2000. J. Biol. Chem.275, 36152.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable