553514-M

RAD51 Inhibitor II, RI-1

The RAD51 Inhibitor, RI-1 controls the biological activity of RAD51. This small molecule/inhibitor is primarily used for Cell Structure applications.

• Synonym(e): RAD51 Inhibitor II, RI-1, 3-chloro-1-(3,4-dichlorophenyl)-4-morpholino-1H-pyrrole-2,5-dione
• Empirische Formel (Hill-System): C₁₄H₁₁Cl₃N₂O₃
• Molekulargewicht: 361.61
• UNSPSC Code: 12352200
• NACRES: NA.28
• Assay: ≥99% (HPLC)
• Form: solid
• Quality level: 100
• Storage condition: OK to freeze, protect from light

Eigenschaften

• Quality Level: 100
• assay: ≥99% (HPLC)
• form: solid
• potency: 5-30 μM IC₅₀
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: bright yellow
• solubility: DMSO: 50 mg/mL
• storage temp.: 2-8°C

Beschreibung

General description

A cell-permeable 1H-Pyrrole-2,3-dione derivative that covalently binds to RAD51 at Cys319 and irreversibly destabilizes a protein-protein interface that is essential for filament formation and recombinase activity. Stimulates single strand annealing at a 5-30 µM concentration. Inhibits subnuclear RAD51 foci formation following DNA damage without affecting replication protein A. Also, effectively sensitizes cancer cells to mitomycin C (~25 µM) and generate synergistic cell kill in RAD51C mutant cells and with lesser effect on RAD51 complemented cells. Satisfies all four Lipinski rules for predicting drug likeness.

Biochem/physiol Actions

Primary Target
RAD51

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Budke, B., et al. 2012. Nucleic Acids Res.40, 7347.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable