538540

MMP-7, Proenzyme, Human, Recombinant, E. coli

• Synonym(e): Promatrilysin, rhPro-MMP-7
• EG-Nummer: 3.4.24.23 (BRENDA, IUBMB)
• NACRES: NA.77
• UNSPSC Code: 12352202
• Specific activity: ≥1400 units/mg protein (after activation)

Eigenschaften

• form: liquid
• Quality Level: 100
• specific activity: ≥1400 units/mg protein (after activation)
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Beschreibung

General description

Recombinant, human pro-MMP-7 expressed in E. coli. MMP-7 is believed to be a key factor in the regulation of metastasis. Overexpression of MMP-7 is observed in colon cancer. Activatable by organomercurials (APMA; Cat. No. 164610).

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 25 mM Tris-HCl, 5 mM CaCl₂, 0.02% NaN₃, 0.01% Brij^® 35 Detergent, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C). Freeze-thaw cycles may accelerate the activation process.

Other Notes

Girolamo, N. D., et al. 2001. Invest. Ophtal. Visual. Sci.42, 1963.
Kihira, Y., et al. 1996. Urol. Oncol. 2, 20.
Woessner, J.F. 1995. Methods Enzymol. 248, 485.
Crabbe, T., et al. 1992. Biochemistry 31, 8500.

One unit is defined as the amount of enzyme that will digest 1.0 µg AZOCOLL Substrate (Cat. No. 194933), in the presence of 500 μM p-aminophenylmercuric acetate (APMA; Cat. No. 164610) per minute at 37°C, pH 7.5.

Legal Information

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable