444282

MMP/TACE Substrate, FS-6

The widely used MMP substrate FS-1 is modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and substrate sensitivities.

• Synonym(e): MMP/TACE Substrate, FS-6, Mca-KPLG~L-Dpa-AR-NH₂
• Empirische Formel (Hill-System): C₅₅H₈₀N₁₆O₁₆
• Molekulargewicht: 1221.32
• NACRES: NA.77
• UNSPSC Code: 12352200
• Assay: ≥97% (HPLC)
• Form: lyophilized
• Storage condition: OK to freeze, desiccated (hygroscopic), protect from light

Eigenschaften

• Quality Segment: 100
• assay: ≥97% (HPLC)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic), protect from light
• solubility: water: 20 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Beschreibung

General description

The widely used MMP substrate FS-1 (Cat. No. 03-32-5032) is modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and substrate sensitivities. Exhibits 2- to 9-fold higher specificity constant (k_cat/K_m) towards MMP-1, -8, -13, -14 when compared with FS-1 and ~2-fold higher k_cat/K_m towards TACE when compared with TACE Substrate II and III (Cat. Nos. 616402 and 616403, respectively). It has been used successfully in detecting T98G glioma cell surface-associated MMP activity and is shown to be resistant to proteolysis by human leukocyte elastase, trypsin, and ADAM-10.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Broad-spectrum MMP and TACE substrate

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Mca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH₂

Neumann, U., et al. 2004. Anal. Biochem.328, 166.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable