444217

MMP-3, Catalytic Domain, Human, Recombinant, E. coli

• Synonym(e): Matrix Metalloproteinase 3, Stromelysin-1, Catalytic Domain
• EG-Nummer: 3.4.24.17 (BRENDA, IUBMB)
• NACRES: NA.77
• UNSPSC Code: 12352202
• Specific activity: ≥1250 mU/mg protein
• Assay: ≥90% (SDS-PAGE)

Eigenschaften

• assay: ≥90% (SDS-PAGE)
• Quality Level: 100
• form: liquid
• specific activity: ≥1250 mU/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• foreign activity: other MMP activity, none detected
• shipped in: wet ice
• storage temp.: −70°C

Beschreibung

General description

Note: 1 mU = 1 milliunit.

Recombinant, human MMP-3 catalytic domain expressed in E. coli.

Physical form

In 50 mM Tris-HCl, 10 mM CaCl₂, 1 µM ZnCl₂, 0.05% NaN₃, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Nagase, H., et al. 1994. J. Biol. Chem. 269, 20952.
Wilhelm, S.M., et al. 1993. J. Biol. Chem. 268, 21906.
Ye, Q.Z., et al. 1992. Biochemistry 31, 11231.

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol MCA-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(DNP)-NH₂ per min at 37°C, pH 7.0.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable