440319

Lumican, His•Tag^®, CF, Mouse, Recombinant

• NACRES: NA.45
• UNSPSC Code: 12352203
• Form: lyophilized
• Assay: ≥95% (SDS-PAGE)

Eigenschaften

• assay: ≥95% (SDS-PAGE)
• Quality Segment: 100
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤1.0 EU/μg protein Endotoxin
• shipped in: ambient
• storage temp.: −20°C

Beschreibung

General description

Recombinant, murine lumican, consisting of amino acids 19-338, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells. Lumincan a 40-kDa member of the small leucine-rich repeat proteoglycans (SLRPs) family/Class II subfamily of proteins. It is known for its ability to modulate collagen fibrillogenesis and role in cell proliferation regulation.

Biochem/physiol Actions

Cell adhesion: 50-70% after 50 min at 37°C as measured by the ability of immobilized lumican to supoprt the adhesion of 5 X 10⁴ BCE C/D-1b bovine cornea-derived endothelial cells.

Physical form

Lyophilized from 0.2 µm filtered PBS.

Preparation Note

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 200 µg/ml with sterile PBS.

Other Notes

D′Onofrio, M.F. et al. 2008. Biochem. Biophys. Res. Commun.365, 266.
Nikitovic, D. et al. 2008. IUBMB Life60, 818.
Nikitovic, D. et al. 2008. FEBS J.275, 350.
Ishiwata, T. et al. 2007. Oncol. Rep.18, 537.
Vuillermoz, B. et al. 2004. Exp. Cell Res.296,294.
Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci.44, 2422.
Jepsen, K.J. et al. 2002. J. Biol. Chem.277, 35532.
Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci.41,3365.
Blochberger, T.C. et al. 1992. J. Biol. Chem.267, 347.
Chakravarti, S. et al. 1998. J. Cell Biol.141, 1277.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 2