393206
Anti-HNE Fluorophore Rabbit pAb
liquid, Calbiochem®
Synonym(e):
Anti-N α-Acetyllysine-4-hydroxy-2-nonenal Fluorophore, Anti-Nα-Acetyllysine-4-hydroxy-2-nonenal Fluorophore
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100 μL
€ 586,00
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100 μL
€ 586,00
About This Item
UNSPSC-Code:
12352203
NACRES:
NA.43
Empfohlene Produkte
Biologische Quelle
rabbit
Qualitätsniveau
Antikörperform
diluted serum
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Form
liquid
Speziesreaktivität (Voraussage durch Homologie)
all
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
OK to freeze
avoid repeated freeze/thaw cycles
Isotyp
IgG
Versandbedingung
wet ice
Lagertemp.
−70°C
Posttranslationale Modifikation Target
unmodified
Allgemeine Beschreibung
Anti-HNE Fluorophore, rabbit polyclonal, recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore. It is validated for use in ELISA, Western blotting, and immunohistochemistry.
Rabbit polyclonal antibody supplied as diluted serum. Recognizes the HNE-derived chromophore of the 2:1 lysine-HNE fluorophore.
Recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore.
Immunogen
a 2:1 Nα-acetyllysine-HNE fluorophore, conjugated to KLH
Anwendung
ELISA (1:500-1:1000)
Immunoblotting (1:1000-1:5000)
Immunohistochemistry (1:100-1:500)
Immunoblotting (1:1000-1:5000)
Immunohistochemistry (1:100-1:500)
Warnhinweis
Toxicity: Standard Handling (A)
Physikalische Form
Serum diluted 1:1 with glycerol.
Rekonstituierung
Following initial thaw, aliquot and freeze (-70°C).
Sonstige Hinweise
Due to the small epitope size, overnight incubation with the antibody greatly increases sensitivity by immunoblotting. Determined by competitive ELISA and immunoblotting to recognize the chromophore structure of fluorophore derived from the reaction of 4-HNE with primary amines. Does not recognize 1:1 cysteine-, 1:1 histidine-, or 1:1 lysine-HNE Michael Adducts. Variables associated with assay conditions will dictate the proper working dilution.
Tsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 7975.
Rechtliche Hinweise
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
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