382129

HIV Reverse Transcriptase, Recombinant, E. coli

liquid, ≥5,000 units/mg protein, Nuclease-free

• Synonym(e): HIV RT
• NACRES: NA.54
• UNSPSC Code: 12352202
• Form: liquid
• Recombinant: expressed in E. coli

Eigenschaften

• recombinant: expressed in E. coli
• Quality Level: 100
• form: liquid
• specific activity: ≥10,000 units/mL, ≥5,000 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• technique(s): molecular cloning: suitable
• input: purified RNA
• foreign activity: Nuclease-free
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

General description

Recombinant HIV reverse transcriptase expressed in E. coli. Catalyzes error-prone synthesis on DNA and RNA templates. Provides an excellent target for evaluating antiviral agents.

Application

HIV Reverse Transcriptase, Recombinant, E.coli has been used as a standard in reverse transcriptase activity assay.[1]

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM potassium phosphate, 1 mM DTT, 20% glycerol, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

One unit is defined as the amount of enzyme that will incorporate 1.0 nmol of labeled dTTP into acid-insoluble material in 20 min at 37°C, pH 8.3.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1