This product is not IVD grade.
324506
EDTA, 0,5 M, pH 8,0, molekularbiologische Qualität, DEPC-behandelt
Sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC). Suitable for use in molecular biology applications.
Synonym(e):
EDTA, 0,5 M, pH 8,0, molekularbiologische Qualität, DEPC-behandelt, Ethylendiamintetraessigsäure-Trinatriumsalz
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 mL | Warenkorb auf Verfügbarkeit prüfen | Anfragen |
Über diesen Artikel
Empirische Formel (Hill-System):
C10H13N2Na3O8 · 3H2O
CAS-Nummer:
Molekulargewicht:
412.23
MDL number:
UNSPSC Code:
12352107
NACRES:
NA.25
Form:
liquid
Grade:
Molecular Biology
Technischer Dienst
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Unterstützung erhaltengrade
Molecular Biology
Quality Segment
form
liquid
reaction suitability
reagent type: chelator
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
pH
8
shipped in
ambient
storage temp.
15-25°C
SMILES string
[Na+].[Na+].[Na+].N(CCN(CC(=O)[O-])CC(=O)O)(CC(=O)[O-])CC(=O)[O-].O.O.O
InChI
1S/C10H16N2O8.3Na.3H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;3*1H2/q;3*+1;;;/p-3
InChI key
FXNQQEVEDZAAJM-UHFFFAOYSA-K
General description
Sterilgefilterte Lösung von 0,5 M EDTA in H2O, behandelt mit Diethyl-Pyrocarbonat (DEPC). Geeignet für molekularbiologische Anwendungen.
Application
- Comparing extraction method efficiency for high-throughput palaeoproteomic bone species identification: In dieser Forschungsarbeit werden verschiedene Extraktionsmethoden für die paläoproteomische Analyse verglichen, einschließlich Methoden, die EDTA verwenden, um Knochenarten effizient zu identifizieren. Die Studie unterstreicht die Bedeutung von EDTA für die Förderung der Proteinextraktion für proteomische Studien (Mylopotamitaki et al., 2023).
- Deep coverage and quantification of the bone proteome provides enhanced opportunities for new discoveries in skeletal biology and disease: Es wird die Verwendung von EDTA bei der Vorbereitung und Extraktion von Knochenproben für die Proteomik-Analyse diskutiert. Dabei wird seine Rolle bei der Erzielung einer tiefen proteomischen Abdeckung und der Ermöglichung neuer Entdeckungen in der Skelettbiologie hervorgehoben (Rose et al., 2023).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxizität: Standard-Handhabung (A)
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Dieser Artikel | |||
|---|---|---|---|
| grade Molecular Biology | grade Molecular Biology | grade - | grade Molecular Biology |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form liquid | form crystalline solid | form liquid | form solid (fine) |
| storage temp. 15-25°C | storage temp. 10-30°C | storage temp. - | storage temp. 10-30°C |
| pH 8 | pH - | pH - | pH - |
| reaction suitability reagent type: chelator | reaction suitability reagent type: chelator | reaction suitability reagent type: chelator | reaction suitability reagent type: chelator |
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - STOT RE 2 Inhalation
target_organs
Respiratory Tract
Lagerklasse
10 - Combustible liquids
wgk
WGK 2
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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is it IVD grade?
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Hi, Is the DEPC innactivated in this solution ? I have some leftover and I am wondering if it would be suitable for use in a cell culture buffer context ? Thank you in advance for your support, Kind regards, Coline
1 answer-
Yes, DEPC was inactivated. Note that one of the break down products of DEPC is ethanol. DEPC-treated solutions are typically not used in cell culture applications and may be cytotoxic.
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Dear all, Is the EDTA 0.5 M solution prepared with before DEPC treated H2O, or, is it prepared together with DEPC and then autoclaved? Many thanks for your answer! Best, Julia
1 answer-
The solution is prepared with DEPC-treated H2O.
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