218799

Caspase-6, Human, Recombinant, E. coli

Caspase-6, Human, Recombinant is expressed as a single polypeptide in E. coli. Fused at the N- and C- termini to a His•Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

• Synonym(e): Mch2
• NACRES: NA.77
• UNSPSC Code: 12352202
• Specific activity: ≥6000 units/mg protein
• Assay: ≥95% (SDS-PAGE)
• Recombinant: expressed in E. coli

Eigenschaften

• recombinant: expressed in E. coli
• Quality Level: 100
• assay: ≥95% (SDS-PAGE)
• form: lyophilized
• specific activity: ≥6000 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Beschreibung

General description

M.W. 11,000 and 18,000 (heterodimer).

Recombinant, human caspase-6 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.

Preparation Note

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.

Reconstitute to 1 unit/µl in PBS, 15% glycerol.

Other Notes

LeBlanc, A., et al. 1999. J. Biol. Chem.274, 23426.
Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem.272, 25719.

One unit is defined as the amount of enzyme that will cleave 1 nmol Ac-VEID-pNA (Cat. No. 218762) per h at 37°C, pH 7.2.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable