1.50435

SeQuant^® ZIC^®-HILIC 200Å (5 µm) HPLC Columns

L × I.D. 20 mm x 2.1 mm Guard Column

• Synonym(e): Hydrophilic Interaction Liquid Chromatography Guard Column
• UNSPSC Code: 41115709
• NACRES: NB.21
• Particle size: 5 μm
• Matrix active group: ZIC-HILIC, ZIC-HILIC bonding phase
• Pore size: 200 Å pore size
• Matrix: fully porous particle

Eigenschaften

• Produktname: SeQuant^® ZIC-HILIC^™ Guard 20 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
• material: PEEK-lined stainless steel column
• Quality Segment: 100
• agency: suitable for USP L114
• product line: SeQuant^®
• feature: endcapped: no
• parameter: 550 bar pressure, 70 °C max. temp.
• technique(s): HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)
• column L × I.D.: 2 cm × 2.1 mm
• matrix: fully porous particle
• matrix active group: ZIC-HILIC, ZIC-HILIC bonding phase
• particle size: 5 μm
• pore size: 200 Å pore size
• operating pH: 1.5-7.5
• separation technique: hydrophilic interaction (HILIC)
• storage temp.: no temp limit

Beschreibung

General description

The SeQuant^® ZIC^®-HILIC Guard column protects your analytical separation column from contamination.
SeQuant^® ZIC^®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility.
The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
Thanks to their favorable retention and robust performance, SeQuant^® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^® ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide

Application

• Development and validation of LC-MS/MS methods to measure tobramycin and lincomycin: Demonstrates the application of SeQuant ZIC-HILIC Guard columns in a pharmacokinetic research study, focusing on the high-precision measurement of pharmaceutical compounds in biological matrices. This method is essential for ensuring accurate dosage and monitoring therapeutic levels, crucial for clinical trials and medical research (Pandey S et al., 2020).

Analysis Note

Extent of testing: conforms

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany