14-730-M

c-Kit (V560G) Protein, active, 10 µg

Active, N-terminal GST tagged recombinant human c-Kit, amino acids 544 -end, containing the V560G mutation, found in patients with gastrointestinal stromal tumours. For use in Kinase Assays.

• Synonym(e): Mast/stem cell growth factor receptor, SCFR, CD117
• UNSPSC Code: 12352202
• NACRES: NA.32
• eCl@ss: 32160405
• Biological source: human
• Mol wt: M_w 76.6 kDa

Eigenschaften

• biological source: human
• Quality Level: 100, 300
• mol wt: M_w 76.6 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NM_000222.2, NM_001093772.1
• UniProt accession no.: P10721
• Gene Information: human ... KIT(3815)

Beschreibung

General description

N-terminal GST tagged recombinant human c-Kit, amino acids 544 - end, containing the V560G mutation. The V560G mutation has been found in patients with gastrointestinal stromal tumours and has been linked to poor prognosis. This mutation confers increased susceptibility to Gleevec.

Product Source: Human c-Kit (V560G) expressed in Sf21 insect cells.

Physical form

Presented as a frozen solution in 50mM Tris/HCl pH 7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, and 0.1% 2-mercaptoethanol.

Analysis Note

Routinely evaluated by phosphorylation of 250μM GGMEDIYFEFMGGKKK.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable