14-429-M

MEK1 Protein, active, 10 µg

Active, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.

• Synonym(e): CFC3, MAP2K1, MAPKK1, MEL, MKK1, PRKMK1, mitogen-activated protein kinase kinase 1
• UNSPSC Code: 12352202
• NACRES: NA.41
• eCl@ss: 32160405
• Form: liquid
• Biological source: human
• Recombinant: expressed in E. coli
• Mol wt: M_w 71 kDa

Eigenschaften

• biological source: human
• Quality Segment: 100
• recombinant: expressed in E. coli
• product line: Upstate^®
• form: liquid
• specific activity: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• shelf life: 1 yr
• mol wt: M_w 71 kDa
• purified by: (Purified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose)
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• solubility: soluble
• accession no.: (FUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. ), NM_002755.2
• NCBI accession no.: NM_002755.2
• UniProt accession no.: Q02750
• storage temp.: −70°C
• Gene Information: human ... MAP2K1(5604)

Beschreibung

General description

Research area: Cell Signaling Protein Target: MEK1 Target Sub-Family: STE

MEK1

N-terminal GST and Cterminal His6-tagged, recombinant full-length human MEK1

Biochem/physiol Actions

Mitogen-activated protein kinase kinase 1 (MEK1) is a hydrophilic non-receptor protein kinase that shows ubiquitous expression across different cell types. It serves as a critical component of the RAS-RAF-MEK-ERK signal transduction cascade, also known as the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) cascade. This signaling pathway plays a crucial role in regulating various biological processes, including apoptosis, cell cycle progression, cell migration, differentiation, metabolism, and proliferation. Additionally, MEK1 protein contributes to the development of the extra-embryonic ectoderm during placentogenesis. Somatic mutations in the MEK1 gene have been observed in individuals with melanoma, a form of skin cancer.

Packaging

Also available in 10 μg size and in bulk

Also available in 250μg size as catalog # 14-429M - Call for pricing and availability.

Physical form

Purified using glutathione agarose followed by Ni2+/NTA agarose, activated using c-Raf and the re-purified using Ni2+/NTA agarose

Preparation Note

1 year at -70°C

Analysis Note

routinely evaluated by phosphorylation of MBP

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_c: Not applicable