12660

Albumin, Bovine Serum, Fraction V, Modified Cohn, pH 5.2

• Synonym(e): Albumin, Bovine Serum, Fraction V, Modified Cohn, pH 5.2
• CAS-Nummer: 9048-46-8
• MDL number: MFCD00064211
• NACRES: NA.25
• UNSPSC Code: 12352202
• eCl@ss: 32160409
• Assay: ≥96% (SDS-PAGE)
• Form: solid
• Impurities: ≤5% Moisture

Eigenschaften

• description: Merck USA index - 14, 8468
• Quality Segment: 100
• assay: ≥96% (SDS-PAGE)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤5% Moisture
• color: off-white
• solubility: 0.15 M sodium chloride: 10 mg/mL
• cation traces: heavy metals: ≤50 ppm
• shipped in: ambient
• storage temp.: 2-8°C

Beschreibung

General description

Albumin, Bovine Serum, Fraction V, Modified Cohn, pH 5.2 is a native fatty acid profile that reflects endogenous lipids. It has a pH in the range of 4.8-5.4 in 150 mM NaCl (1%).

Bovine serum albumin (BSA), a non-glycosylated globular, α-helical protein, belongs to the serum albumin family. It consists of three domains with two sub-domains each and has 17-disulfide bonds.[1][2]

Application

Albumin, Bovine Serum, Fraction V, Modified Cohn, pH 5.2 has been used as a blocking agent in western blotting.[3] It has also been used as a component in phosphate-buffered saline (PBS) for diluting secondary antibodies and blocking in immunofluorescence to observe the localization of the Golgi apparatus.[4]

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the most vital component of cell culture media. It aids in the differentiation of embryonic stem cells (hESC).[5] It plays a key role in the transportation of drugs, hormones, and fatty acids.[1] BSA is used in enzyme-linked immunosorbent assay (ELISA) as a blocking agent.[6]

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable