1.25072

Fractogel^® EMD SO₃ ⁻ (M) Harz

MiniChrom Column Fractogel^® SO3 (M), volume 5 mL, prepacked column

• Synonym(e): MiniChrom Column Fractogel^® SO3 (M), 5 ml
• UNSPSC Code: 41115711

Eigenschaften

• ligand: (Sulfoisobutyl)
• material: polypropylene housing
• product line: Fractogel^®
• form: prepacked column
• parameter: ≤8 bar pressure, ~200 cm/hr max. flow rate
• column I.D. × L: 8 mm × 100 mm
• volume: 5 mL
• matrix active group: Methacrylate
• mean particle size: 40-90 μm
• capacity: ~130 mL, resin binding capacity
• application(s): mAb; recombinant protein; vaccine development
• separation technique: cation exchange
• storage temp.: 15-25°C

Beschreibung

General description

8 x 100mm

Features and Benefits

MiniChrom Column Fractogel^® SO3 (M), 5 ml enables:

• Increased binding strength at low pH
• High aggregate removal efficiency combined with high dynamic binding capacity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel^® EMD SO₃^- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Packaging

1 pkg Plastic bag(s)

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany