1.01374

Brilliant green (hydrogen sulfate) (C.I. 42040)

for microscopy Certistain^®

• Synonym(e): Ethylgrün, Diamantgrün G, Malachitgrün G
• Empirische Formel (Hill-System): C₂₇H₃₄N₂O₄S
• CAS-Nummer: 633-03-4
• Molekulargewicht: 482.63
• MDL-Nummer: MFCD00011880
• UNSPSC-Code: 41116130
• EG-Index-Nummer: 211-190-1

Eigenschaften

• Qualitätsniveau: 400
• Form: solid
• Wirksamkeit: 300-400 mg/kg LD₅₀, oral (Rat)
• Zusammensetzung: Dye, ≥95% spectrophotometric assay
• pH-Wert: 2.5 (25 °C, 10 g/L in H₂O)
• mp (Schmelzpunkt): 210 °C (decomposition)
• Löslichkeit: 100 g/L
• Schüttdichte: 500‑600 kg/m³
• εmax: 2210-2350 at 628-632 nm in 50% ethanol at 0.003 g/L
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: 2-30°C
• InChI: 1S/C27H33N2.H2O4S/c1-5-28(6-2)25-18-14-23(15-19-25)27(22-12-10-9-11-13-22)24-16-20-26(21-17-24)29(7-3)8-4;1-5(2,3)4/h9-21H,5-8H2,1-4H3;(H2,1,2,3,4)/q+1;/p-1
• InChIKey: NNBFNNNWANBMTI-UHFFFAOYSA-M

Beschreibung

Allgemeine Beschreibung

Brilliant green (hydrogen sulfate) (C.I. 42040) for microscopy Certistain^®, is a dry staining dye that is used to prepare a staining solution makes target structures in bacteriological specimen materials evaluable for diagnostic purposes. In the area of bacteriology, among others the Gram staining method, the Ziehl-Neelsen staining method, and the spore-staining method according to Rakette are used to yield a differentiated image of pathogens. This can be done either by using eosin Y as a counterstain or by modification according to Wirtz using safranine O solution. By this method, spores are always stained green and the cell elements always red.
As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Hinweis zur Analyse

Farbstoffgehalt (spektralphotometrisch): ≥ 95 %
Identität
- Hydrogensulfat (als Schwefel):
entspricht
- UV/VIS-Spektrum: entspricht
Absorptionsmaximum λ max. (Ethanol 50 %): 628 - 632 nm
Spez. Extinktion E1%/1cm (λ_max; 0.003 g/l; Ethanol 50 %): 2210 - 2350
DC-Prüfung: entspricht
Trocknungsverlust (110 °C): ≤ 5 %
Eignung für die Mikroskopie: entspricht

Rechtliche Hinweise

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H302 - H319
• P-Sätze: P264 - P270 - P280 - P301 + P312 - P305 + P351 + P338 - P337 + P313
• Gefahreneinstufungen: Acute Tox. 4 Oral - Eye Irrit. 2
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°C): Not applicable